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当前位置:五分时时彩 > 产品中心 > 五分时时彩:生化试剂、抗体、血清 > 试剂盒 > 12381QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒

QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒
名称 QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒
型号 12381
报价 1
特点 QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒
EndoFree Plasmid Kits基于阴离子交换技术,可快速制备不含内毒素的质粒DNA。QIAfilter Cartridges过滤快速澄清裂解液。制备的DNA纯度超过两次CsCl梯度离心法所得DNA的纯度,适合超转染级纯的应用。EndoFree Plasmid Buffer Set可用于制备10次m}
  • 详细内容

QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒

纯化得到至多10 mg的无内毒素高转染级纯质粒或柯斯质粒DNA

  • 纯化得到的DNA内毒素水平低于0.1 EU/µg
  • 纯化流程快,同时去除内毒素
  • 获得高产量、高拷贝质粒DNA
  • 使用LyseBlue,获得zui好的裂解和高产量的DNA

QIAGEN EndoFree Plasmid Mega Kit无内毒素质粒提取试剂盒

质粒纯化方法与转染效率。

使用两种方法独立制备pRSVcat,分别使用脂质体介导的方法转染到COS-7、HeLa和LMH细胞中,及使用磷酸钙转染至Huh7细胞中。平均转染效率以相对于QIAGEN Plasmid Kit(100%)制备的DNA的转染效率表示。

质粒纯化方法与转染效率。

使用两种方法独立制备pRSVcat,分别使用脂质体介导的方法转染到COS-7、HeLa和LMH细胞中,及使用磷酸钙转染至Huh7细胞中。平均转染效率以相对于QIAGEN Plasmid Kit(100%)制备的DNA的转染效率表示。

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